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Chapter title |
Detecting Protein-Protein Interactions by Gel Filtration Chromatography
|
---|---|
Chapter number | 13 |
Book title |
Protein-Protein Interactions
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2425-7_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2424-0, 978-1-4939-2425-7
|
Authors |
Yan Bai, Bai, Yan |
Abstract |
Upon protein-protein interaction, the formed complex is subject to a change in size. A number of methods can be utilized to detect such a change. Gel filtration technology is well recognized for its ability to monitor and separate protein species of different sizes, and can greatly facilitate functional studies of protein complexes. In addition, gel filtration can be performed in any buffer system that preserves the protein complex formation and function. Therefore, it can be a significantly useful method for studying protein interactions. In this chapter, a protocol for performing gel filtration is described in detail. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 7 | 22% |
Student > Master | 6 | 19% |
Student > Ph. D. Student | 4 | 13% |
Other | 2 | 6% |
Student > Doctoral Student | 2 | 6% |
Other | 4 | 13% |
Unknown | 7 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 38% |
Agricultural and Biological Sciences | 5 | 16% |
Chemistry | 2 | 6% |
Computer Science | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Other | 1 | 3% |
Unknown | 10 | 31% |