Chapter title |
Functionality Testing of Primary Hepatocytes in Culture by Measuring Urea Synthesis
|
---|---|
Chapter number | 24 |
Book title |
Protocols in In Vitro Hepatocyte Research
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2074-7_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
Authors |
Jennifer Bolleyn, Vera Rogiers, Tamara Vanhaecke, Bolleyn, Jennifer, Rogiers, Vera, Vanhaecke, Tamara |
Abstract |
One of the mechanisms of healthy hepatocytes to detoxify ammonia in the liver consists of converting it into urea. When liver function is impaired, this detoxification capacity decreases and may cause severe pathologies, such as hepatic encephalopathy. Consequently, urea synthesis is a parameter that can be used to monitor liver functionality. In this chapter, a protocol for the measurement of urea synthesis in the culture medium of cultured rat hepatocytes is described. The procedure relies on a chromogenic reagent that specifically forms a colored complex with urea. The latter can be measured colorimetrically and is directly proportional to the urea concentration in the sample. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 23% |
Student > Master | 3 | 12% |
Researcher | 3 | 12% |
Student > Doctoral Student | 2 | 8% |
Student > Bachelor | 1 | 4% |
Other | 2 | 8% |
Unknown | 9 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 15% |
Biochemistry, Genetics and Molecular Biology | 3 | 12% |
Chemistry | 3 | 12% |
Medicine and Dentistry | 2 | 8% |
Psychology | 1 | 4% |
Other | 2 | 8% |
Unknown | 11 | 42% |