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Protocols in In Vitro Hepatocyte Research

Overview of attention for book
Cover of 'Protocols in In Vitro Hepatocyte Research'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments
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    Chapter 2 Cryopreservation of Hepatocytes
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    Chapter 3 Protocols in In Vitro Hepatocyte Research
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    Chapter 4 Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
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    Chapter 5 Culture and Functional Characterization of Human Hepatoma HepG2 Cells
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    Chapter 6 Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death
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    Chapter 7 Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes
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    Chapter 8 Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation
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    Chapter 9 Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling.
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    Chapter 10 Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation
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    Chapter 11 Coculture and Long-Term Maintenance of Hepatocytes.
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    Chapter 12 Primary Hepatocytes in Sandwich Culture
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    Chapter 13 Establishing Liver Bioreactors for In Vitro Research
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    Chapter 14 Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture.
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    Chapter 15 Transfection of Primary Hepatocytes with Liver-Enriched Transcription Factors Using Adenoviral Vectors
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    Chapter 16 Transcriptomics of Hepatocytes Treated with Toxicants for Investigating Molecular Mechanisms Underlying Hepatotoxicity.
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    Chapter 17 Global MicroRNA Analysis in Primary Hepatocyte Cultures.
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    Chapter 18 Mass Spectrometry-Based Proteomics for Relative Protein Quantification and Biomarker Identification in Primary Human Hepatocytes
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    Chapter 19 Targeted Metabolomics for Homocysteine-Related Metabolites in Primary Hepatocytes
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    Chapter 20 Measurement of Cytochrome P450 Enzyme Induction and Inhibition in Human Hepatoma Cells
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    Chapter 21 Analysis of Sinusoidal Drug Uptake Transporter Activities in Primary Human Hepatocytes
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    Chapter 22 Measurement of Albumin Secretion as Functionality Test in Primary Hepatocyte Cultures
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    Chapter 23 Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes
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    Chapter 24 Functionality Testing of Primary Hepatocytes in Culture by Measuring Urea Synthesis
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    Chapter 25 Protocols in In Vitro Hepatocyte Research
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    Chapter 26 General Cytotoxicity Assessment by Means of the MTT Assay
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    Chapter 27 Measurement of Apoptotic and Necrotic Cell Death in Primary Hepatocyte Cultures
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    Chapter 28 Critical Factors in the Assessment of Cholestatic Liver Injury In Vitro
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    Chapter 29 In Vitro Cell Culture Models of Hepatic Steatosis
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    Chapter 30 Assessment of Liver Fibrotic Insults In Vitro
Attention for Chapter 5: Culture and Functional Characterization of Human Hepatoma HepG2 Cells
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

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Citations

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Chapter title
Culture and Functional Characterization of Human Hepatoma HepG2 Cells
Chapter number 5
Book title
Protocols in In Vitro Hepatocyte Research
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2074-7_5
Pubmed ID
26272135
Book ISBNs
978-1-4939-2073-0, 978-1-4939-2074-7
Authors

María Teresa Donato, Laia Tolosa, María José Gómez-Lechón, Donato, María Teresa, Tolosa, Laia, Gómez-Lechón, María José

Abstract

Hepatoma cell lines are frequently used as in vitro alternatives to primary human hepatocytes. Cell lines are characterized by their unlimited life span, stable phenotype, high availability, and easy handling. However, their major limitation is the lower expression of some metabolic activities compared with hepatocytes. HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions. In this chapter, freezing, thawing, and subculturing procedures for HepG2 cells are described. We further provide protocols for evaluating lipid accumulation, glycogen storage, urea synthesis, and phase I and phase II drug metabolizing activities in HepG2 cells.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 518 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 518 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 113 22%
Student > Ph. D. Student 63 12%
Student > Master 55 11%
Researcher 32 6%
Student > Doctoral Student 27 5%
Other 40 8%
Unknown 188 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 118 23%
Pharmacology, Toxicology and Pharmaceutical Science 53 10%
Agricultural and Biological Sciences 43 8%
Chemistry 26 5%
Engineering 15 3%
Other 59 11%
Unknown 204 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2023.
All research outputs
#3,085,665
of 23,659,844 outputs
Outputs from Methods in molecular biology
#634
of 13,344 outputs
Outputs of similar age
#44,161
of 356,430 outputs
Outputs of similar age from Methods in molecular biology
#40
of 988 outputs
Altmetric has tracked 23,659,844 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,344 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,430 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 988 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.

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