Chapter title |
General Cytotoxicity Assessment by Means of the MTT Assay
|
---|---|
Chapter number | 26 |
Book title |
Protocols in In Vitro Hepatocyte Research
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2074-7_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
Authors |
Laia Tolosa, María Teresa Donato, María José Gómez-Lechón, Tolosa, Laia, Donato, María Teresa, Gómez-Lechón, María José |
Abstract |
Cytotoxicity assays were among the first in vitro bioassay methods used to predict toxicity of substances to various tissues. In vitro cytotoxicity testing provides a crucial means for safety assessment and screening, and for ranking compounds. The choice of using a particular cytotoxicity assay technology may be influenced by specific research goals. As such, four main classes of assays are used to monitor the response of cultured cells after treatment with potential toxicants. These methods measure viability, cell membrane integrity, cell proliferation, and metabolic activity. In this chapter, we focus on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction colorimetric assay to evaluate detrimental intracellular effects on metabolic activity. This assay is well-characterized, simple to use and remains popular in several laboratories worldwide. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 286 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 43 | 15% |
Student > Master | 32 | 11% |
Student > Ph. D. Student | 24 | 8% |
Researcher | 23 | 8% |
Lecturer | 10 | 3% |
Other | 26 | 9% |
Unknown | 128 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 38 | 13% |
Chemistry | 28 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 21 | 7% |
Medicine and Dentistry | 18 | 6% |
Agricultural and Biological Sciences | 12 | 4% |
Other | 34 | 12% |
Unknown | 135 | 47% |