| Chapter title |
Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation
|
|---|---|
| Chapter number | 10 |
| Book title |
Protocols in In Vitro Hepatocyte Research
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2074-7_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
| Authors |
Varvara Gouliarmou, Olavi Pelkonen, Sandra Coecke, Gouliarmou, Varvara, Pelkonen, Olavi, Coecke, Sandra |
| Abstract |
Isolated primary hepatocytes are considered as the reference system for in vitro hepatic methods. Following the isolation of primary hepatocytes from liver tissue, an unfavorable process named dedifferentiation is initiated leading to the attenuation of the hepatocellular phenotype both at the morphological and functional level. Freshly isolated hepatocytes can be used immediately or can be cryopreserved for future purposes. Currently, a number of antidedifferentiation strategies exist to extend the life span of isolated hepatocytes. The addition of differentiation-promoting compounds to the hepatocyte culture medium is the oldest and simplest antidedifferentiation approach applied. In the present chapter, the most commonly used medium additives for cultivation and cryopreservation of primary hepatocytes are reviewed. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 27% |
| Student > Ph. D. Student | 3 | 20% |
| Professor | 1 | 7% |
| Other | 1 | 7% |
| Student > Master | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 4 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 27% |
| Agricultural and Biological Sciences | 3 | 20% |
| Nursing and Health Professions | 1 | 7% |
| Social Sciences | 1 | 7% |
| Engineering | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 5 | 33% |