| Chapter title |
Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
|
|---|---|
| Chapter number | 4 |
| Book title |
Protocols in In Vitro Hepatocyte Research
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2074-7_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
| Authors |
Eva Ramboer, Tamara Vanhaecke, Vera Rogiers, Mathieu Vinken, Ramboer, Eva, Vanhaecke, Tamara, Rogiers, Vera, Vinken, Mathieu |
| Abstract |
The ubiquitous shortage of primary human hepatocytes has urged the scientific community to search for alternative cell sources, such as immortalized hepatic cell lines. Over the years, several human hepatic cell lines have been produced, whether or not using a combination of viral oncogenes and human telomerase reverse transcriptase protein. Conditional approaches for hepatocyte immortalization have also been established and allow generation of growth-controlled cell lines. A variety of immortalized human hepatocytes have already proven useful as tools for liver-based in vitro testing and fundamental research purposes. The present chapter describes currently applied immortalization strategies and provides an overview of the actually available immortalized human hepatic cell lines and their in vitro applications. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | <1% |
| Unknown | 107 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 26 | 24% |
| Researcher | 19 | 18% |
| Student > Master | 18 | 17% |
| Student > Bachelor | 9 | 8% |
| Student > Doctoral Student | 5 | 5% |
| Other | 9 | 8% |
| Unknown | 22 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 38 | 35% |
| Agricultural and Biological Sciences | 18 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 9 | 8% |
| Medicine and Dentistry | 7 | 6% |
| Immunology and Microbiology | 3 | 3% |
| Other | 6 | 6% |
| Unknown | 27 | 25% |