Chapter title |
Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes
|
---|---|
Chapter number | 23 |
Book title |
Protocols in In Vitro Hepatocyte Research
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2074-7_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
Authors |
Stefan Heinz, Joris Braspenning, Heinz, Stefan, Braspenning, Joris |
Abstract |
An important function of the liver is the synthesis and secretion of blood coagulation factors. Within the liver, hepatocytes are involved in the synthesis of most blood coagulation factors, such as fibrinogen, prothrombin, factor V, VII, IX, X, XI, XII, as well as protein C and S, and antithrombin, whereas liver sinusoidal endothelial cells produce factor VIII and von Willebrand factor. Here, we describe methods for the detection and quantification of most blood coagulation factors in hepatocytes in vitro. Hepatocyte cultures indeed provide a valuable tool to study blood coagulation factors. In addition, the generation and expansion of hepatocytes or hepatocyte-like cells may be used in future for cell-based therapies of liver diseases, including blood coagulation factor deficiencies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 53 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 12 | 23% |
Student > Ph. D. Student | 8 | 15% |
Student > Doctoral Student | 4 | 8% |
Student > Master | 3 | 6% |
Other | 1 | 2% |
Other | 5 | 9% |
Unknown | 20 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 15 | 28% |
Biochemistry, Genetics and Molecular Biology | 6 | 11% |
Veterinary Science and Veterinary Medicine | 3 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
Agricultural and Biological Sciences | 1 | 2% |
Other | 5 | 9% |
Unknown | 20 | 38% |