| Chapter title |
Primary Hepatocytes in Sandwich Culture
|
|---|---|
| Chapter number | 12 |
| Book title |
Protocols in In Vitro Hepatocyte Research
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2074-7_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2073-0, 978-1-4939-2074-7
|
| Authors |
Janneke Keemink, Marlies Oorts, Pieter Annaert, Keemink, Janneke, Oorts, Marlies, Annaert, Pieter |
| Abstract |
Hepatocytes in sandwich configuration constitute of primary hepatocytes cultured between two layers of extracellular matrix. Sandwich-cultured hepatocytes maintain expression of liver-specific proteins and gradually form intact bile canaliculi with functional biliary excretion of endogenous compounds and xenobiotics. Both freshly isolated and cryopreserved hepatocytes can be used to establish sandwich cultures. Therefore, this preclinical model has become an invaluable in vitro tool to evaluate hepatobiliary drug transport, metabolism, hepatotoxicity, and drug interactions. In this chapter, commonly used procedures to cultivate primary hepatocytes from human and rat in sandwich configuration are described. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 38% |
| Student > Doctoral Student | 5 | 13% |
| Researcher | 5 | 13% |
| Student > Master | 5 | 13% |
| Professor | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 5 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 12 | 30% |
| Agricultural and Biological Sciences | 8 | 20% |
| Biochemistry, Genetics and Molecular Biology | 4 | 10% |
| Medicine and Dentistry | 4 | 10% |
| Chemistry | 3 | 8% |
| Other | 3 | 8% |
| Unknown | 6 | 15% |