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High Throughput Screening

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Cover of 'High Throughput Screening'

Table of Contents

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    Book Overview
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    Chapter 1 Design and Implementation of High-Throughput Screening Assays
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    Chapter 2 Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method
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    Chapter 3 High Throughput Screening
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    Chapter 4 Structure-Based Virtual Screening of Commercially Available Compound Libraries
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    Chapter 5 AlphaScreen-Based Assays: Ultra-High-Throughput Screening for Small-Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions
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    Chapter 6 Instrument Quality Control
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    Chapter 7 Application of Fluorescence Polarization in HTS Assays
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    Chapter 8 Time-Resolved Fluorescence Assays
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    Chapter 9 Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays
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    Chapter 10 Screening for Inhibitors of Kinase Autophosphorylation
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    Chapter 11 A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
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    Chapter 12 High Throughput Screening
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    Chapter 13 Identification of State-Dependent Blockers for Voltage-Gated Calcium Channels Using a FLIPR-Based Assay
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    Chapter 14 A Luciferase Reporter Gene System for High-Throughput Screening of γ -Globin Gene Activators
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    Chapter 15 A High-Throughput Flow Cytometry Assay for Identification of Inhibitors of 3′,5′-Cyclic Adenosine Monophosphate Efflux
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    Chapter 16 High-Throughput Cell Toxicity Assays
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    Chapter 17 BRET: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells
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    Chapter 18 Application of Imaging-Based Assays in Microplate Formats for High-Content Screening
Attention for Chapter 7: Application of Fluorescence Polarization in HTS Assays
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Chapter title
Application of Fluorescence Polarization in HTS Assays
Chapter number 7
Book title
High Throughput Screening
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3673-1_7
Pubmed ID
Book ISBNs
978-1-4939-3671-7, 978-1-4939-3673-1
Authors

Xinyi Huang, Ann Aulabaugh, Huang, Xinyi, Aulabaugh, Ann

Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 3%
United States 1 3%
Denmark 1 3%
Unknown 32 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 29%
Student > Ph. D. Student 8 23%
Student > Doctoral Student 3 9%
Professor > Associate Professor 3 9%
Student > Master 3 9%
Other 5 14%
Unknown 3 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 40%
Chemistry 7 20%
Agricultural and Biological Sciences 5 14%
Physics and Astronomy 1 3%
Environmental Science 1 3%
Other 2 6%
Unknown 5 14%