| Chapter title |
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
|
|---|---|
| Chapter number | 11 |
| Book title |
High Throughput Screening
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3673-1_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3671-7, 978-1-4939-3673-1
|
| Authors |
Thomas M. Smith, Daryl L. Richie, Jianshi Tao, Smith, Thomas M., Richie, Daryl L., Tao, Jianshi |
| Abstract |
Due to the advancements in modern medicine that have resulted in an increased number of immunocompromised individuals, the incidences and the associated mortality of invasive aspergillosis have continued to rise over the past three decades despite appropriate treatment. As a result, invasive aspergillosis has emerged as a leading cause of infection-related mortality in immunocompromised individuals. Utilizing the resazurin to resorufin conversion fluorescence readout to monitor cell viability, herein, we outline a high-throughput screening method amenable to profiling a large pharmaceutical library against the clinically relevant but less frequently screened fungal pathogen Aspergillus fumigatus. This enables the user to conduct high-throughput screening using a disease-relevant fungal growth assay and identify novel antifungal chemotypes as drug leads. |
Mendeley readers
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|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 29% |
| Other | 1 | 14% |
| Lecturer > Senior Lecturer | 1 | 14% |
| Student > Bachelor | 1 | 14% |
| Researcher | 1 | 14% |
| Other | 0 | 0% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 1 | 14% |