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High Throughput Screening

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Cover of 'High Throughput Screening'

Table of Contents

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    Book Overview
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    Chapter 1 Design and Implementation of High-Throughput Screening Assays
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    Chapter 2 Characterization of Inhibitor Binding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method
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    Chapter 3 High Throughput Screening
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    Chapter 4 Structure-Based Virtual Screening of Commercially Available Compound Libraries
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    Chapter 5 AlphaScreen-Based Assays: Ultra-High-Throughput Screening for Small-Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions
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    Chapter 6 Instrument Quality Control
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    Chapter 7 Application of Fluorescence Polarization in HTS Assays
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    Chapter 8 Time-Resolved Fluorescence Assays
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    Chapter 9 Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays
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    Chapter 10 Screening for Inhibitors of Kinase Autophosphorylation
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    Chapter 11 A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
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    Chapter 12 High Throughput Screening
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    Chapter 13 Identification of State-Dependent Blockers for Voltage-Gated Calcium Channels Using a FLIPR-Based Assay
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    Chapter 14 A Luciferase Reporter Gene System for High-Throughput Screening of γ -Globin Gene Activators
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    Chapter 15 A High-Throughput Flow Cytometry Assay for Identification of Inhibitors of 3′,5′-Cyclic Adenosine Monophosphate Efflux
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    Chapter 16 High-Throughput Cell Toxicity Assays
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    Chapter 17 BRET: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells
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    Chapter 18 Application of Imaging-Based Assays in Microplate Formats for High-Content Screening
Attention for Chapter 6: Instrument Quality Control
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Chapter title
Instrument Quality Control
Chapter number 6
Book title
High Throughput Screening
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3673-1_6
Pubmed ID
Book ISBNs
978-1-4939-3671-7, 978-1-4939-3673-1
Authors

Chatura Jayakody, Emily A. Hull-Ryde, Jayakody, Chatura, Hull-Ryde, Emily A.

Abstract

Well-defined quality control (QC) processes are used to determine whether a certain procedure or action conforms to a widely accepted standard and/or set of guidelines, and are important components of any laboratory quality assurance program (Popa-Burke et al., J Biomol Screen 14: 1017-1030, 2009). In this chapter, we describe QC procedures useful for monitoring the accuracy and precision of laboratory instrumentation, most notably automated liquid dispensers. Two techniques, gravimetric QC and photometric QC, are highlighted in this chapter. When used together, these simple techniques provide a robust process for evaluating liquid handler accuracy and precision, and critically underpin high-quality research programs.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 50%
Unknown 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Medicine and Dentistry 1 50%