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Yeast Genetics

Overview of attention for book
Cover of 'Yeast Genetics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.
  3. Altmetric Badge
    Chapter 2 Tetrad, random spore, and molecular analysis of meiotic segregation and recombination.
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    Chapter 3 PCR Mutagenesis and Gap Repair in Yeast
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    Chapter 4 PCR-Mediated Epitope Tagging of Genes in Yeast.
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    Chapter 5 Manipulating the yeast genome: deletion, mutation, and tagging by PCR.
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    Chapter 6 Preparation of yeast cells for live-cell imaging and indirect immunofluorescence.
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    Chapter 7 Single yeast cell imaging.
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    Chapter 8 Microfluidic platforms for generating dynamic environmental perturbations to study the responses of single yeast cells.
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    Chapter 9 Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations.
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    Chapter 10 Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast
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    Chapter 11 Chemical genetic and chemogenomic analysis in yeast.
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    Chapter 12 Phenomic assessment of genetic buffering by kinetic analysis of cell arrays.
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    Chapter 13 Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast.
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    Chapter 14 Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.
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    Chapter 15 Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq.
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    Chapter 16 High-density tiling microarray analysis of the full transcriptional activity of yeast.
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    Chapter 17 Analysis of Silencing in Saccharomyces cerevisiae.
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    Chapter 18 A User's Guide to the Ribosomal DNA in Saccharomyces cerevisiae.
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    Chapter 19 Two-dimensional agarose gel electrophoresis for analysis of DNA replication.
  21. Altmetric Badge
    Chapter 20 Replicative life span analysis in budding yeast.
  22. Altmetric Badge
    Chapter 21 Metabolomic and lipidomic analyses of chronologically aging yeast.
Attention for Chapter 1: Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.
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About this Attention Score

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  • High Attention Score compared to outputs of the same age and source (83rd percentile)

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4 patents

Citations

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225 Mendeley
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Chapter title
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.
Chapter number 1
Book title
Yeast Genetics
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1363-3_1
Pubmed ID
Book ISBNs
978-1-4939-1362-6, 978-1-4939-1363-3
Authors

R Daniel Gietz, R. Daniel Gietz, Gietz, R. Daniel

Abstract

Efficient transformation of yeast has been the corner stone of molecular studies in yeast for numerous years. It is essential for techniques such as the yeast two-hybrid system as well as genome modification. Here I present protocols for a number of different transformation applications. A quick and easy protocol is for situations in which large numbers of transformants are not required. The high-efficiency protocol generates large numbers of transformants from small amounts of DNA that can be scaled up for the library transformation protocol. A transformation protocol for a microtiter plate format is also included. Finally, a protocol for the production of frozen competent yeast cells is included, which allows cells to be taken from the freezer at a moment's notice and transformed with high efficiency.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 225 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Sweden 1 <1%
Argentina 1 <1%
Unknown 222 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 63 28%
Student > Master 41 18%
Researcher 28 12%
Student > Bachelor 26 12%
Student > Postgraduate 10 4%
Other 23 10%
Unknown 34 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 101 45%
Agricultural and Biological Sciences 62 28%
Engineering 8 4%
Chemistry 5 2%
Immunology and Microbiology 4 2%
Other 7 3%
Unknown 38 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2022.
All research outputs
#7,538,708
of 25,837,817 outputs
Outputs from Methods in molecular biology
#2,284
of 14,362 outputs
Outputs of similar age
#81,367
of 322,344 outputs
Outputs of similar age from Methods in molecular biology
#92
of 572 outputs
Altmetric has tracked 25,837,817 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 14,362 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 322,344 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 572 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.