Chapter title |
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.
|
---|---|
Chapter number | 1 |
Book title |
Yeast Genetics
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1363-3_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1362-6, 978-1-4939-1363-3
|
Authors |
R Daniel Gietz, R. Daniel Gietz, Gietz, R. Daniel |
Abstract |
Efficient transformation of yeast has been the corner stone of molecular studies in yeast for numerous years. It is essential for techniques such as the yeast two-hybrid system as well as genome modification. Here I present protocols for a number of different transformation applications. A quick and easy protocol is for situations in which large numbers of transformants are not required. The high-efficiency protocol generates large numbers of transformants from small amounts of DNA that can be scaled up for the library transformation protocol. A transformation protocol for a microtiter plate format is also included. Finally, a protocol for the production of frozen competent yeast cells is included, which allows cells to be taken from the freezer at a moment's notice and transformed with high efficiency. |
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