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Yeast Genetics

Overview of attention for book
Cover of 'Yeast Genetics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.
  3. Altmetric Badge
    Chapter 2 Tetrad, random spore, and molecular analysis of meiotic segregation and recombination.
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    Chapter 3 PCR Mutagenesis and Gap Repair in Yeast
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    Chapter 4 PCR-Mediated Epitope Tagging of Genes in Yeast.
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    Chapter 5 Manipulating the yeast genome: deletion, mutation, and tagging by PCR.
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    Chapter 6 Preparation of yeast cells for live-cell imaging and indirect immunofluorescence.
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    Chapter 7 Single yeast cell imaging.
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    Chapter 8 Microfluidic platforms for generating dynamic environmental perturbations to study the responses of single yeast cells.
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    Chapter 9 Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations.
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    Chapter 10 Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast
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    Chapter 11 Chemical genetic and chemogenomic analysis in yeast.
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    Chapter 12 Phenomic assessment of genetic buffering by kinetic analysis of cell arrays.
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    Chapter 13 Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast.
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    Chapter 14 Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.
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    Chapter 15 Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq.
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    Chapter 16 High-density tiling microarray analysis of the full transcriptional activity of yeast.
  18. Altmetric Badge
    Chapter 17 Analysis of Silencing in Saccharomyces cerevisiae.
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    Chapter 18 A User's Guide to the Ribosomal DNA in Saccharomyces cerevisiae.
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    Chapter 19 Two-dimensional agarose gel electrophoresis for analysis of DNA replication.
  21. Altmetric Badge
    Chapter 20 Replicative life span analysis in budding yeast.
  22. Altmetric Badge
    Chapter 21 Metabolomic and lipidomic analyses of chronologically aging yeast.
Attention for Chapter 14: Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.
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Chapter title
Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.
Chapter number 14
Book title
Yeast Genetics
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1363-3_14
Pubmed ID
Book ISBNs
978-1-4939-1362-6, 978-1-4939-1363-3
Authors

Maria D Mayán, Luis Aragón, Maria D. Mayán, Mayán, Maria D., Aragón, Luis

Abstract

Studying interphase chromosome arrangements at the molecular level can provide important details on the function and coordination of many metabolic processes that take place on DNA, such as transcription or DNA repair. The chromosome conformation capture (3C) methodology was originally developed in yeast to study the interphase organization of a single-yeast chromosome. 3C assays allow the identification of physical interactions between distant DNA segments and thus provide detailed information on the folding of chromatin in the native cellular state. Since its initial development, the technique has been used to advance our understanding of the folding of gene loci and has yielded important discoveries, like the demonstration that distant transcriptional regulatory elements interact with their target promoters through chromatin loops. The 3C method uses formaldehyde cross-linking to covalently link interacting chromatin segments in intact cells. After chromatin digestion and ligation of cross-linked fragments, the frequency of interaction between distant DNA elements can be quantified. However, the design, analysis, and controls used are critical when using 3C. In this chapter, we describe the general protocol for 3C analysis and demonstrate it through analysis of interactions in repetitive sequences of the ribosomal gene array of Saccharomyces cerevisiae.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 7%
Unknown 14 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 33%
Student > Ph. D. Student 3 20%
Student > Master 2 13%
Student > Bachelor 1 7%
Professor > Associate Professor 1 7%
Other 0 0%
Unknown 3 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 47%
Biochemistry, Genetics and Molecular Biology 5 33%
Materials Science 1 7%
Unknown 2 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2014.
All research outputs
#15,117,322
of 23,262,131 outputs
Outputs from Methods in molecular biology
#4,807
of 13,319 outputs
Outputs of similar age
#185,705
of 307,642 outputs
Outputs of similar age from Methods in molecular biology
#186
of 591 outputs
Altmetric has tracked 23,262,131 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,319 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,642 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 591 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.