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Mendeley readers
Attention Score in Context
Chapter title |
PCR Mutagenesis and Gap Repair in Yeast
|
---|---|
Chapter number | 3 |
Book title |
Yeast Genetics
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1363-3_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1362-6, 978-1-4939-1363-3
|
Authors |
Mark Weir, Jill B Keeney, Jill B. Keeney, Weir, Mark, Keeney, Jill B. |
Abstract |
Random mutagenesis of a gene and subsequent screening for phenotypic properties is a basic genetic procedure for studying gene function. When working with yeast, cloning mutated alleles into a yeast vector can be done directly due to the efficiency of homologous recombination. Here we give sample protocols for introducing random mutations to a gene using PCR and transformation of the resulting product into yeast. After screening resultant colonies for the desired phenotype, the plasmid containing the selected mutation is easily rescued to bacteria for sequencing. A reliable protocol for rescuing yeast plasmids to bacteria is given. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 12% |
Unknown | 15 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 29% |
Student > Ph. D. Student | 5 | 29% |
Student > Bachelor | 4 | 24% |
Student > Doctoral Student | 1 | 6% |
Professor | 1 | 6% |
Other | 0 | 0% |
Unknown | 1 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 53% |
Agricultural and Biological Sciences | 7 | 41% |
Unknown | 1 | 6% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2014.
All research outputs
#14,518,338
of 23,262,131 outputs
Outputs from Methods in molecular biology
#4,282
of 13,319 outputs
Outputs of similar age
#176,307
of 307,642 outputs
Outputs of similar age from Methods in molecular biology
#169
of 591 outputs
Altmetric has tracked 23,262,131 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,319 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,642 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 591 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.