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Yeast Genetics

Overview of attention for book
Cover of 'Yeast Genetics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.
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    Chapter 2 Tetrad, random spore, and molecular analysis of meiotic segregation and recombination.
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    Chapter 3 PCR Mutagenesis and Gap Repair in Yeast
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    Chapter 4 PCR-Mediated Epitope Tagging of Genes in Yeast.
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    Chapter 5 Manipulating the yeast genome: deletion, mutation, and tagging by PCR.
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    Chapter 6 Preparation of yeast cells for live-cell imaging and indirect immunofluorescence.
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    Chapter 7 Single yeast cell imaging.
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    Chapter 8 Microfluidic platforms for generating dynamic environmental perturbations to study the responses of single yeast cells.
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    Chapter 9 Using Two-Hybrid Interactions to Identify Separation-of-Function Mutations.
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    Chapter 10 Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast
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    Chapter 11 Chemical genetic and chemogenomic analysis in yeast.
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    Chapter 12 Phenomic assessment of genetic buffering by kinetic analysis of cell arrays.
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    Chapter 13 Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast.
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    Chapter 14 Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast.
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    Chapter 15 Global Analysis of Transcription Factor-Binding Sites in Yeast Using ChIP-Seq.
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    Chapter 16 High-density tiling microarray analysis of the full transcriptional activity of yeast.
  18. Altmetric Badge
    Chapter 17 Analysis of Silencing in Saccharomyces cerevisiae.
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    Chapter 18 A User's Guide to the Ribosomal DNA in Saccharomyces cerevisiae.
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    Chapter 19 Two-dimensional agarose gel electrophoresis for analysis of DNA replication.
  21. Altmetric Badge
    Chapter 20 Replicative life span analysis in budding yeast.
  22. Altmetric Badge
    Chapter 21 Metabolomic and lipidomic analyses of chronologically aging yeast.
Attention for Chapter 8: Microfluidic platforms for generating dynamic environmental perturbations to study the responses of single yeast cells.
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Chapter title
Microfluidic platforms for generating dynamic environmental perturbations to study the responses of single yeast cells.
Chapter number 8
Book title
Yeast Genetics
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-1363-3_8
Pubmed ID
Book ISBNs
978-1-4939-1362-6, 978-1-4939-1363-3
Authors

Anjali Bisaria, Pascal Hersen, Megan N McClean, Megan N. McClean, Bisaria, Anjali, Hersen, Pascal, McClean, Megan N.

Abstract

Microfluidic platforms are ideal for generating dynamic temporal and spatial perturbations in extracellular environments. Single cells and organisms can be trapped and maintained in microfluidic platforms for long periods of time while their responses to stimuli are measured using appropriate fluorescence reporters and time-lapse microscopy. Such platforms have been used to study problems as diverse as C. elegans olfaction (Chronis et al. Nature Methods 4:727-731, 2007), cancer cell migration (Huang et al. Biomicrofluidics 5:13412, 2011), and E. coli chemotaxis (Ahmed et al. Integr Biol 2:604-629, 2010). In this paper we describe how to construct and use a microfluidic chip to study the response of single yeast cells to dynamic perturbations of their fluid environment. The method involves creation of a photoresist master mold followed by subsequent creation of a polydimethylsiloxane (PDMS) microfluidic chip for maintaining live yeast cells in a channel with two inputs for stimulating the cells. We emphasize simplicity and the methods discussed here are accessible to the average biological laboratory. We cover the basic toolbox for making microfluidic lab-on-a-chip devices, and the techniques discussed serve as a starting point for creating sophisticated microfluidic devices capable of implementing more complicated experimental protocols.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 31%
Student > Master 3 19%
Student > Bachelor 2 13%
Student > Ph. D. Student 1 6%
Unspecified 1 6%
Other 2 13%
Unknown 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 44%
Biochemistry, Genetics and Molecular Biology 2 13%
Arts and Humanities 1 6%
Unspecified 1 6%
Medicine and Dentistry 1 6%
Other 1 6%
Unknown 3 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 September 2014.
All research outputs
#15,651,328
of 23,262,131 outputs
Outputs from Methods in molecular biology
#5,463
of 13,319 outputs
Outputs of similar age
#192,569
of 307,642 outputs
Outputs of similar age from Methods in molecular biology
#202
of 591 outputs
Altmetric has tracked 23,262,131 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,319 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,642 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 591 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.