| Chapter title |
Manipulating the yeast genome: deletion, mutation, and tagging by PCR.
|
|---|---|
| Chapter number | 5 |
| Book title |
Yeast Genetics
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-1363-3_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1362-6, 978-1-4939-1363-3
|
| Authors |
Jennifer M Gardner, Sue L Jaspersen, Jennifer M. Gardner, Sue L. Jaspersen, Gardner, Jennifer M., Jaspersen, Sue L. |
| Abstract |
Saccharomyces cerevisiae is an ideal model eukaryotic system for the systematic analysis of gene function due to the ease and precision with which its genome can be manipulated. The ability of budding yeast to undergo efficient homologous recombination with short stretches of sequence homology has led to an explosion of PCR-based methods to delete and mutate yeast genes and to create fusions to epitope tags and fluorescent proteins. Here, we describe commonly used methods to generate gene deletions, to integrate mutated versions of a gene into the yeast genome, and to construct N- and C-terminal gene fusions. Using a high-efficiency yeast transformation protocol, DNA fragments with as little as 40 bp of homology can accurately target integration into a particular region of the yeast genome. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Israel | 1 | <1% |
| Spain | 1 | <1% |
| Unknown | 240 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 53 | 22% |
| Student > Bachelor | 40 | 17% |
| Student > Master | 30 | 12% |
| Researcher | 27 | 11% |
| Student > Doctoral Student | 12 | 5% |
| Other | 27 | 11% |
| Unknown | 53 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 124 | 51% |
| Agricultural and Biological Sciences | 35 | 14% |
| Chemistry | 10 | 4% |
| Immunology and Microbiology | 4 | 2% |
| Environmental Science | 3 | 1% |
| Other | 10 | 4% |
| Unknown | 56 | 23% |