Report for: Cancer Epigenetics

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Chapter title	Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR).
Chapter number	18
Book title	Cancer Epigenetics
Published in	Methods in molecular biology, January 2012
DOI	10.1007/978-1-61779-612-8_18
Pubmed ID	22359301
Book ISBNs	978-1-61779-611-1, 978-1-61779-612-8
Authors	Richard N. Armstrong, Hilary A. A. Colyer, Ken I. Mills, Armstrong, Richard N., Colyer, Hilary A. A., Mills, Ken I.
Abstract	In this section, we describe the use of Applied Biosystems TaqMan Array microRNA Card Set 3.0 to identify miRNA expression in a given RNA sample. This array set includes an array "A" and an array "B" which each have 384 wells that contain specific forward, reverse, and probe oligoinucleotides for measuring the expression of individual miRNAs during a Real-Time PCR (Q-PCR) reaction. Array "A" includes assays for profiling the comparatively higher expressed and better characterized miRNAs. Presently there are 1,048 mature miRNAs annotated in miRBase (release 16). The relatively small amount of miRNAs in comparison to protein-coding genes makes this format a viable option for measuring genome-wide miRNA expression changes. The Applied Biosystems TaqMan miRNA array set 3.0, which includes two separate arrays, can profile 754 miRNAs.

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Geographical breakdown

Country	Count	As %
Australia	1	100%

Demographic breakdown

Type	Count	As %
Scientists	1	100%

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
United Kingdom	1	8%
Unknown	11	92%

Demographic breakdown

Readers by professional status	Count	As %
Student > Ph. D. Student	2	17%
Researcher	2	17%
Student > Postgraduate	2	17%
Student > Master	2	17%
Other	1	8%
Other	3	25%

Readers by discipline	Count	As %
Agricultural and Biological Sciences	6	50%
Medicine and Dentistry	2	17%
Biochemistry, Genetics and Molecular Biology	1	8%
Immunology and Microbiology	1	8%
Unspecified	1	8%
Other	0	0%
Unknown	1	8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 February 2012.

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#18,304,874

of 22,663,150 outputs

Outputs from Methods in molecular biology

#7,811

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#195,921

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Outputs of similar age from Methods in molecular biology

#325

of 473 outputs

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Cancer Epigenetics

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