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Cancer Epigenetics

Overview of attention for book
Cover of 'Cancer Epigenetics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Epigenetic Markers of Early Tumor Development
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    Chapter 2 Epigenetics of Solid Cancer Stem Cells
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    Chapter 3 DNA Methylation and Histone Modifications in Breast Cancer
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    Chapter 4 DNA Methylation Changes in Prostate Cancer
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    Chapter 5 DNA methylation in promoter region as biomarkers in prostate cancer.
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    Chapter 6 Epigenetics of Bladder Cancer
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    Chapter 7 Epigenetics in Myeloid Malignancies
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    Chapter 8 The Epigenetics of Brain Tumors
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    Chapter 9 DNA Methylation Changes in Cervical Cancers
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    Chapter 10 Epigenetics of colon cancer.
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    Chapter 11 Promoter Methylation in Head and Neck Tumorigenesis
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    Chapter 12 Epigenome and DNA Methylation in Oral Squamous Cell Carcinoma
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    Chapter 13 Clinical Implications of Epigenetic Alterations in Human Thoracic Malignancies: Epigenetic Alterations in Lung Cancer
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    Chapter 14 The Role of MicroRNAs in the Management of Liver Cancer.
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    Chapter 15 Epigenetics in ovarian cancer.
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    Chapter 16 DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification
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    Chapter 17 Detection and analysis of DNA methylation by pyrosequencing.
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    Chapter 18 Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR).
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    Chapter 19 Genome-Wide Methylation Analysis
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    Chapter 20 Microarray for Epigenetic Changes: Gene Expression Arrays
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    Chapter 21 Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique
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    Chapter 22 Nutrigenomics: implications for breast and colon cancer prevention.
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    Chapter 23 Dietary and lifestyle factors of DNA methylation.
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    Chapter 24 Diet, epigenetics, and cancer.
  26. Altmetric Badge
    Chapter 25 Role of Epigenetics in Cancer Health Disparities
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    Chapter 26 Multifactorial Etiology of Gastric Cancer
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    Chapter 27 Epigenetic Epidemiology for Cancer Risk: Harnessing Germline Epigenetic Variation
  29. Altmetric Badge
    Chapter 28 Epigenetic Biomarkers in Cancer Epidemiology
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    Chapter 29 Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR)
Attention for Chapter 17: Detection and analysis of DNA methylation by pyrosequencing.
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Chapter title
Detection and analysis of DNA methylation by pyrosequencing.
Chapter number 17
Book title
Cancer Epigenetics
Published in
Methods in molecular biology, February 2012
DOI 10.1007/978-1-61779-612-8_17
Pubmed ID
Book ISBNs
978-1-61779-611-1, 978-1-61779-612-8
Authors

Colyer HA, Armstrong RN, Sharpe DJ, Mills KI, Hilary A. A. Colyer, Richard N. Armstrong, Daniel J. Sharpe, Ken I. Mills, Colyer, Hilary A. A., Armstrong, Richard N., Sharpe, Daniel J., Mills, Ken I.

Abstract

Pyrosequencing is a "sequencing by synthesis" technique which can be used to quantify DNA methylation at specific CpG sites within the target region of interest. Biotin labelled polymerase chain reaction (PCR) products form the template for base-pair nucleotide incorporation causing a light emitting cascade reaction resulting in the formation of a pyrogram and the calculation of the percentage methylation for each site. Prior to pyrosequencing, it is essential to bisulphite-convert the DNA sample and then perform locus-specific PCR for the region of interest. One of the PCR primers needs to be biotinylated and a separate sequencing primer is required for the pyrosequencing itself.

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 1 11%
Unknown 8 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Researcher 2 22%
Professor 2 22%
Student > Doctoral Student 1 11%
Student > Master 1 11%
Other 1 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 56%
Biochemistry, Genetics and Molecular Biology 3 33%
Medicine and Dentistry 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 February 2013.
All research outputs
#15,349,970
of 17,367,552 outputs
Outputs from Methods in molecular biology
#6,845
of 9,919 outputs
Outputs of similar age
#135,579
of 157,785 outputs
Outputs of similar age from Methods in molecular biology
#17
of 23 outputs
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So far Altmetric has tracked 9,919 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.