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Bacterial Transcriptional Control

Overview of attention for book
Cover of 'Bacterial Transcriptional Control'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III
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    Chapter 2 Purification of Bacterial RNA Polymerase: Tools and Protocols
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    Chapter 3 Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues
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    Chapter 4 In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.
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    Chapter 5 Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems.
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    Chapter 6 Site-Specific Incorporation of Probes into RNA Polymerase by Unnatural-Amino-Acid Mutagenesis and Staudinger–Bertozzi Ligation
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    Chapter 7 Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts.
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    Chapter 8 Direct Competition Assay for Transcription Fidelity
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    Chapter 9 Single-Stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase
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    Chapter 10 Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.
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    Chapter 11 Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments
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    Chapter 12 Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends.
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    Chapter 13 In Situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde
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    Chapter 14 Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation
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    Chapter 15 Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-Termination Decisions In Vitro
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    Chapter 16 Purification of active RNA polymerase I from yeast.
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    Chapter 17 Transcription in Archaea: Preparation of Methanocaldococcus jannaschii Transcription Machinery.
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    Chapter 18 Transcription in Archaea: In Vitro Transcription Assays for mjRNAP.
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    Chapter 19 Experimental Analysis of hFACT Action During Pol II Transcription In Vitro
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    Chapter 20 ChIP-Seq for Genome-Scale Analysis of Bacterial DNA-Binding Proteins.
Attention for Chapter 5: Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems.
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  • Above-average Attention Score compared to outputs of the same age and source (57th percentile)

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Chapter title
Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems.
Chapter number 5
Book title
Bacterial Transcriptional Control
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2392-2_5
Pubmed ID
25665559
Book ISBNs
978-1-4939-2391-5, 978-1-4939-2392-2
Authors

Daniel Castro-Roa, Nikolay Zenkin, Castro-Roa, Daniel, Zenkin, Nikolay

Abstract

RNA polymerase is a complex machinery, which is further embedded in interactions with other cellular components that interplay with either the transcribed DNA (DNA polymerases, topoisomerases, etc.) or the nascent RNA (RNA processing enzymes, ribosomes, etc.). In prokaryotes, coupling of transcription and translation is thought to play many regulatory roles but the mechanistic understanding of their interactions has been hindered by the lack of a defined experimental system. Here, we describe a pure transcription-coupled-to-translation system in which control of the ribosome has been achieved through its stepwise translocation towards RNA polymerase. This system can be used to study the effects of concurrent translation on RNA chain elongation and to elucidate the interface between the two macromolecular complexes.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 25%
Student > Ph. D. Student 2 25%
Unspecified 1 13%
Professor > Associate Professor 1 13%
Lecturer > Senior Lecturer 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 38%
Biochemistry, Genetics and Molecular Biology 2 25%
Unspecified 1 13%
Unknown 2 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2015.
All research outputs
#15,322,159
of 22,789,076 outputs
Outputs from Methods in molecular biology
#5,324
of 13,096 outputs
Outputs of similar age
#208,865
of 352,988 outputs
Outputs of similar age from Methods in molecular biology
#343
of 996 outputs
Altmetric has tracked 22,789,076 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,096 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,988 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.

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