Chapter title |
Transcription in Archaea: In Vitro Transcription Assays for mjRNAP.
|
---|---|
Chapter number | 18 |
Book title |
Bacterial Transcriptional Control
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2392-2_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2391-5, 978-1-4939-2392-2
|
Authors |
Katherine Smollett, Fabian Blombach, Finn Werner, Smollett, Katherine, Blombach, Fabian, Werner, Finn |
Abstract |
The fully recombinant Methanocaldococcus jannaschii RNA polymerase allows for a detailed dissection of the different stages of the transcription. In the previous chapter, we discussed how to purify the different components of the M. jannaschii transcription system, the RNA polymerase subunits, and general transcription factors and how to assemble a functional M. jannaschii enzyme. Standard in vitro transcription assays can be used to examine the different stages of transcription. In this chapter, we describe how some of these assays have been optimized for M. jannaschii RNA polymerase, which transcribes at much higher temperatures than many other transcription complexes. |
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