Chapter title |
Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends.
|
---|---|
Chapter number | 12 |
Book title |
Bacterial Transcriptional Control
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2392-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2391-5, 978-1-4939-2392-2
|
Authors |
Irina O Vvedenskaya, Seth R Goldman, Bryce E Nickels, Irina O. Vvedenskaya, Seth R. Goldman, Bryce E. Nickels, Vvedenskaya, Irina O., Goldman, Seth R., Nickels, Bryce E. |
Abstract |
We provide a detailed protocol for preparing cDNA libraries suitable for high-throughput sequencing that are derived specifically from the 5' ends of RNA (5' specific RNA-seq). The protocol describes how cDNA libraries for 5' specific RNA-seq can be tailored to analyze specific classes of RNAs based upon the phosphorylation status of the 5' end. Thus, the analysis of cDNA libraries generated by these methods provides information regarding both the sequence and phosphorylation status of the 5' ends of RNAs. 5' specific RNA-seq can be used to analyze transcription initiation and posttranscriptional processing of RNAs with single base pair resolution on a genome-wide level. |
Mendeley readers
Geographical breakdown
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 3 | 11% |
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Unspecified | 2 | 7% |
Professor | 1 | 4% |
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Unknown | 9 | 33% |
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Unknown | 9 | 33% |