| Chapter title |
Transcription in Archaea: Preparation of Methanocaldococcus jannaschii Transcription Machinery.
|
|---|---|
| Chapter number | 17 |
| Book title |
Bacterial Transcriptional Control
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2392-2_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2391-5, 978-1-4939-2392-2
|
| Authors |
Katherine Smollett, Fabian Blombach, Finn Werner, Smollett, Katherine, Blombach, Fabian, Werner, Finn |
| Abstract |
Archaeal RNA polymerase and general transcription factors are more closely related to those of eukaryotes than of bacteria. As such the study of transcription of archaea is important both in terms of examination of the evolution of the transcriptional machinery and as a simplified tool for eukaryotic transcription. In particular, the hyperthermophilic Methanocaldococcus jannaschii provides us with a fully recombinant RNA polymerase system allowing for much more detailed in vitro examination of the roles of different components during the transcription cycle than otherwise possible. The individual subunits of M. jannaschii enzyme are easily expressed and purified from heterologous expression systems. Forming functional RNA polymerase involves simply combining the different subunits under denaturing conditions and slowly removing the denaturant. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 8% |
| Unknown | 12 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 3 | 23% |
| Student > Ph. D. Student | 3 | 23% |
| Researcher | 2 | 15% |
| Student > Doctoral Student | 1 | 8% |
| Student > Master | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 46% |
| Agricultural and Biological Sciences | 4 | 31% |
| Unknown | 3 | 23% |