Chapter title |
In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.
|
---|---|
Chapter number | 4 |
Book title |
Bacterial Transcriptional Control
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2392-2_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2391-5, 978-1-4939-2392-2
|
Authors |
Martin Buck, Christoph Engl, Nicolas Joly, Goran Jovanovic, Milija Jovanovic, Edward Lawton, Christopher McDonald, Jörg Schumacher, Christopher Waite, Nan Zhang, Buck, Martin, Engl, Christoph, Joly, Nicolas, Jovanovic, Goran, Jovanovic, Milija, Lawton, Edward, McDonald, Christopher, Schumacher, Jörg, Waite, Christopher, Zhang, Nan |
Abstract |
Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ(54)), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ(54)-dependent gene expression. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 27% |
Student > Ph. D. Student | 2 | 18% |
Other | 1 | 9% |
Lecturer | 1 | 9% |
Professor | 1 | 9% |
Other | 3 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 55% |
Chemical Engineering | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Unknown | 3 | 27% |