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Bacterial Transcriptional Control

Overview of attention for book
Cover of 'Bacterial Transcriptional Control'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III
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    Chapter 2 Purification of Bacterial RNA Polymerase: Tools and Protocols
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    Chapter 3 Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues
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    Chapter 4 In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.
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    Chapter 5 Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems.
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    Chapter 6 Site-Specific Incorporation of Probes into RNA Polymerase by Unnatural-Amino-Acid Mutagenesis and Staudinger–Bertozzi Ligation
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    Chapter 7 Reconstitution of factor-dependent, promoter proximal pausing in Drosophila nuclear extracts.
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    Chapter 8 Direct Competition Assay for Transcription Fidelity
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    Chapter 9 Single-Stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase
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    Chapter 10 Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae.
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    Chapter 11 Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments
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    Chapter 12 Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends.
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    Chapter 13 In Situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde
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    Chapter 14 Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation
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    Chapter 15 Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-Termination Decisions In Vitro
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    Chapter 16 Purification of active RNA polymerase I from yeast.
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    Chapter 17 Transcription in Archaea: Preparation of Methanocaldococcus jannaschii Transcription Machinery.
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    Chapter 18 Transcription in Archaea: In Vitro Transcription Assays for mjRNAP.
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    Chapter 19 Experimental Analysis of hFACT Action During Pol II Transcription In Vitro
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    Chapter 20 ChIP-Seq for Genome-Scale Analysis of Bacterial DNA-Binding Proteins.
Attention for Chapter 4: In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.
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Chapter title
In vitro and in vivo methodologies for studying the sigma 54-dependent transcription.
Chapter number 4
Book title
Bacterial Transcriptional Control
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2392-2_4
Pubmed ID
25665558
Book ISBNs
978-1-4939-2391-5, 978-1-4939-2392-2
Authors

Martin Buck, Christoph Engl, Nicolas Joly, Goran Jovanovic, Milija Jovanovic, Edward Lawton, Christopher McDonald, Jörg Schumacher, Christopher Waite, Nan Zhang, Buck, Martin, Engl, Christoph, Joly, Nicolas, Jovanovic, Goran, Jovanovic, Milija, Lawton, Edward, McDonald, Christopher, Schumacher, Jörg, Waite, Christopher, Zhang, Nan

Abstract

Here we describe approaches and methods to assaying in vitro the major variant bacterial sigma factor, Sigma 54 (σ(54)), in a purified system. We include the complete transcription system, binding interactions between σ54 and its activators, as well as the self-assembly and the critical ATPase activity of the cognate activators which serve to remodel the closed promoter complexes. We also present in vivo methodologies that are used to study the impact of physiological processes, metabolic states, global signalling networks, and cellular architecture on the control of σ(54)-dependent gene expression.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 27%
Student > Ph. D. Student 2 18%
Other 1 9%
Lecturer 1 9%
Professor 1 9%
Other 3 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 55%
Chemical Engineering 1 9%
Agricultural and Biological Sciences 1 9%
Unknown 3 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 October 2015.
All research outputs
#20,259,335
of 22,789,076 outputs
Outputs from Methods in molecular biology
#9,890
of 13,096 outputs
Outputs of similar age
#295,704
of 352,984 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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