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Mendeley readers
| Chapter title |
Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos
|
|---|---|
| Chapter number | 9 |
| Book title |
Transgenic Ascidians
|
| Published in |
Advances in experimental medicine and biology, January 2018
|
| DOI | 10.1007/978-981-10-7545-2_9 |
| Pubmed ID | |
| Book ISBNs |
978-9-81-107544-5, 978-9-81-107545-2
|
| Authors |
Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen |
| Abstract |
Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 42% |
| Professor > Associate Professor | 2 | 17% |
| Unspecified | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Unknown | 3 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 33% |
| Unspecified | 1 | 8% |
| Biochemistry, Genetics and Molecular Biology | 1 | 8% |
| Social Sciences | 1 | 8% |
| Unknown | 5 | 42% |