Chapter title |
CRISPR Knockouts in Ciona Embryos
|
---|---|
Chapter number | 13 |
Book title |
Transgenic Ascidians
|
Published in |
Advances in experimental medicine and biology, January 2018
|
DOI | 10.1007/978-981-10-7545-2_13 |
Pubmed ID | |
Book ISBNs |
978-9-81-107544-5, 978-9-81-107545-2
|
Authors |
Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi |
Abstract |
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage. |
X Demographics
Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 25% |
Researcher | 3 | 11% |
Student > Bachelor | 2 | 7% |
Other | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Other | 4 | 14% |
Unknown | 8 | 29% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 9 | 32% |
Agricultural and Biological Sciences | 6 | 21% |
Neuroscience | 2 | 7% |
Social Sciences | 1 | 4% |
Unspecified | 1 | 4% |
Other | 2 | 7% |
Unknown | 7 | 25% |