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Mendeley readers
| Chapter title |
TALEN-Based Knockout System
|
|---|---|
| Chapter number | 12 |
| Book title |
Transgenic Ascidians
|
| Published in |
Advances in experimental medicine and biology, January 2018
|
| DOI | 10.1007/978-981-10-7545-2_12 |
| Pubmed ID | |
| Book ISBNs |
978-9-81-107544-5, 978-9-81-107545-2
|
| Authors |
Keita Yoshida, Nicholas Treen |
| Abstract |
Targeted mutagenesis of genes-of-interest is a powerful method of addressing the functions of genes. Genome editing techniques, such as transcriptional activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 systems, have enabled this approach in various organisms because of their ease of use. In the ascidian, Ciona intestinalis, recent studies show that TALEN-based knockout can be applied to establishing both mutant lines and tissue-specific knockout for addressing gene functions. Here, we introduce recent updates to the TALEN toolkit that facilitate detailed functional analysis of genes in ascidians. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 17% |
| Researcher | 2 | 17% |
| Student > Ph. D. Student | 1 | 8% |
| Unspecified | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 5 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 25% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Unspecified | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Unknown | 5 | 42% |