| Chapter title |
Allelic Exchange.
|
|---|---|
| Chapter number | 187 |
| Book title |
The Genetic Manipulation of Staphylococci
|
| Published in |
Methods in molecular biology, February 2015
|
| DOI | 10.1007/7651_2014_187 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3157-6, 978-1-4939-3158-3
|
| Authors |
McKenzie K Lehman, Jeffrey L Bose, Kenneth W Bayles, McKenzie K. Lehman, Jeffrey L. Bose, Kenneth W. Bayles, Lehman, McKenzie K., Bose, Jeffrey L., Bayles, Kenneth W. |
| Abstract |
Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 20% |
| Student > Bachelor | 5 | 14% |
| Student > Master | 5 | 14% |
| Professor > Associate Professor | 3 | 9% |
| Student > Doctoral Student | 2 | 6% |
| Other | 4 | 11% |
| Unknown | 9 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 37% |
| Immunology and Microbiology | 4 | 11% |
| Agricultural and Biological Sciences | 4 | 11% |
| Medicine and Dentistry | 2 | 6% |
| Environmental Science | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 9 | 26% |