Chapter title |
Three-Dimensional Co-Culture Bioassay for Screening of Retinal Gene Delivery Systems
|
---|---|
Chapter number | 6 |
Book title |
Retinal Gene Therapy
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7522-8_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7521-1, 978-1-4939-7522-8
|
Authors |
Ding Wen Chen, Kathleen Pauloff, Marianna Foldvari |
Abstract |
Herein we describe a three-dimensional co-culture bioassay protocol designed to assess the therapeutic potential of the proteins expressed from gene delivery transfected cells through the evaluation of expressed protein bioavailability and bioactivity. Using a combination of enzyme-linked immunosorbent assay (ELISA) and immunofluorescent-based neurite length profiling methodologies, the bioavailability of the secreted therapeutic protein in the medium can be quantitated, and the bioactivity of the secreted therapeutic protein can also be evaluated through neurite length profiling, respectively. The versatility and rationale of this bioassay could serve as a useful screening tool in the development of retinal gene delivery systems. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Professor | 1 | 17% |
Student > Bachelor | 1 | 17% |
Other | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 2 | 33% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 17% |
Agricultural and Biological Sciences | 1 | 17% |
Medicine and Dentistry | 1 | 17% |
Unknown | 1 | 17% |