Chapter title |
CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology
|
---|---|
Chapter number | 13 |
Book title |
Retinal Gene Therapy
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7522-8_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7521-1, 978-1-4939-7522-8
|
Authors |
Wen-Hsuan Wu, Yi-Ting Tsai, Sally Justus, Galaxy Y. Cho, Jesse D. Sengillo, Yu Xu, Thiago Cabral, Chyuan-Sheng Lin, Alexander G. Bassuk, Vinit B. Mahajan, Stephen H. Tsang, Wu, Wen-Hsuan, Tsai, Yi-Ting, Justus, Sally, Cho, Galaxy Y., Sengillo, Jesse D., Xu, Yu, Cabral, Thiago, Lin, Chyuan-Sheng, Bassuk, Alexander G., Mahajan, Vinit B., Tsang, Stephen H. |
Abstract |
CRISPR/Cas9 genome engineering is currently the leading genome surgery technology in most genetics laboratories. Combined with other complementary techniques, it serves as a powerful tool for uncovering genotype-phenotype correlations. Here, we describe a simplified protocol that was used in our publication, CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa, providing an overview of each section of the experimental process. |
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 26 | 100% |
Demographic breakdown
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Student > Bachelor | 6 | 23% |
Professor | 4 | 15% |
Researcher | 2 | 8% |
Student > Doctoral Student | 1 | 4% |
Other | 1 | 4% |
Other | 2 | 8% |
Unknown | 10 | 38% |
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Medicine and Dentistry | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Other | 3 | 12% |
Unknown | 11 | 42% |