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Retinal Gene Therapy

Overview of attention for book
Cover of 'Retinal Gene Therapy'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Small Scale Production of Recombinant Adeno-Associated Viral Vectors for Gene Delivery to the Nervous System
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    Chapter 2 Small and Micro-Scale Recombinant Adeno-Associated Virus Production and Purification for Ocular Gene Therapy Applications
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    Chapter 3 Design and Development of AAV-based Gene Supplementation Therapies for Achromatopsia and Retinitis Pigmentosa
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    Chapter 4 Development of Multigenic Lentiviral Vectors for Cell-Specific Expression of Antiangiogenic miRNAs and Protein Factors
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    Chapter 5 Design and In Vitro Use of Antisense Oligonucleotides to Correct Pre-mRNA Splicing Defects in Inherited Retinal Dystrophies
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    Chapter 6 Three-Dimensional Co-Culture Bioassay for Screening of Retinal Gene Delivery Systems
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    Chapter 7 Retinal Gene Therapy for Choroideremia: In Vitro Testing for Gene Augmentation Using an Adeno-Associated Viral (AAV) Vector
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    Chapter 8 In Vivo Electroporation of Developing Mouse Retina
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    Chapter 9 Methods for In Vivo CRISPR/Cas Editing of the Adult Murine Retina
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    Chapter 10 AAV Gene Augmentation Therapy for CRB1-Associated Retinitis Pigmentosa
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    Chapter 11 Dual AAV Vectors for Stargardt Disease
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    Chapter 12 Optogenetic Retinal Gene Therapy with the Light Gated GPCR Vertebrate Rhodopsin
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    Chapter 13 CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology
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    Chapter 14 In-Depth Functional Analysis of Rodents by Full-Field Electroretinography
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    Chapter 15 Advanced Ocular Injection Techniques for Therapy Approaches
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    Chapter 16 Neutralizing Antibodies Against Adeno-Associated Virus (AAV): Measurement and Influence on Retinal Gene Delivery
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    Chapter 17 Screening for Neutralizing Antibodies Against Natural and Engineered AAV Capsids in Nonhuman Primate Retinas
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    Chapter 18 Subretinal and Intravitreal Retinal Injections in Monkeys
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    Chapter 19 Production of iPS-Derived Human Retinal Organoids for Use in Transgene Expression Assays
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    Chapter 20 AAV Serotype Testing on Cultured Human Donor Retinal Explants
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    Chapter 21 Human Retinal Explant Culture for Ex Vivo Validation of AAV Gene Therapy
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    Chapter 22 Visual Acuity Testing Before and After Intravitreal Injection of rAAV2-ND4 in Patients
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    Chapter 23 Recording and Analysis of the Human Clinical Electroretinogram
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    Chapter 24 Recording and Analysis of Goldmann Kinetic Visual Fields
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    Chapter 25 Measuring Central Retinal Sensitivity Using Microperimetry
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    Chapter 26 Inspection of the Human Retina by Optical Coherence Tomography
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    Chapter 27 Vector Shedding and Immunogenicity Sampling for Retinal Gene Therapy
Attention for Chapter 19: Production of iPS-Derived Human Retinal Organoids for Use in Transgene Expression Assays
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  • High Attention Score compared to outputs of the same age and source (88th percentile)

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Chapter title
Production of iPS-Derived Human Retinal Organoids for Use in Transgene Expression Assays
Chapter number 19
Book title
Retinal Gene Therapy
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7522-8_19
Pubmed ID
29188520
Book ISBNs
978-1-4939-7521-1, 978-1-4939-7522-8
Authors

Peter M. Quinn, Thilo M. Buck, Charlotte Ohonin, Harald M. M. Mikkers, Jan Wijnholds, Quinn, Peter M., Buck, Thilo M., Ohonin, Charlotte, Mikkers, Harald M. M., Wijnholds, Jan

Abstract

In vitro retinal organoid modeling from human pluripotent stem cells is becoming more common place in many ophthalmic laboratories worldwide. These organoids mimic human retinogenesis through formation of organized layered retinal structures that display markers for typical retinal cell types. Pivotally these humanized retinal models provide a stepping stone to the clinic as therapeutic tools and are expected to provide a promising alternative to current animal models. Thus pluripotent stem cell based healthy as well as diseased human retinal organoids are attractive for use in drug potency assays and gene augmentation therapeutics. Here we outline an established protocol for generation of these retinal organoids and how they can be used in conjunction with adeno-associated virus vectors for transgene expression assays.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 21%
Researcher 7 17%
Student > Bachelor 5 12%
Student > Master 5 12%
Student > Doctoral Student 3 7%
Other 6 14%
Unknown 7 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 26%
Neuroscience 8 19%
Medicine and Dentistry 4 10%
Agricultural and Biological Sciences 3 7%
Engineering 2 5%
Other 4 10%
Unknown 10 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2020.
All research outputs
#6,214,272
of 23,009,818 outputs
Outputs from Methods in molecular biology
#1,841
of 13,157 outputs
Outputs of similar age
#125,434
of 442,310 outputs
Outputs of similar age from Methods in molecular biology
#162
of 1,498 outputs
Altmetric has tracked 23,009,818 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,310 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.

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