Chapter title |
Development of Multigenic Lentiviral Vectors for Cell-Specific Expression of Antiangiogenic miRNAs and Protein Factors
|
---|---|
Chapter number | 4 |
Book title |
Retinal Gene Therapy
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7522-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7521-1, 978-1-4939-7522-8
|
Authors |
Anne Louise Askou, Thomas J. Corydon |
Abstract |
Generation of lentivirus (LV)-based vectors holding multiple gene cassettes for coexpression of several therapeutic factors provides potent tools in both gene delivery studies as well as in gene therapy. Here we describe the development of such multigenic LV gene delivery vectors enabling cell-specific coexpression of antiangiogenic microRNA (miRNA) and protein factors and, if preferred, a fluorescent reporter, from RNApol(II)-driven expression cassettes orientated in a back-to-back fashion. This configuration may contribute to the development of new combination therapies for amelioration of diseases involving intraocular neovascularization including exudative age-related macular degeneration (AMD). |
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Unknown | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
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Student > Doctoral Student | 3 | 33% |
Student > Ph. D. Student | 2 | 22% |
Student > Master | 1 | 11% |
Unknown | 3 | 33% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Agricultural and Biological Sciences | 2 | 22% |
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Medicine and Dentistry | 1 | 11% |
Unknown | 3 | 33% |