Chapter title |
Mapping DNA Breaks by Next-Generation Sequencing
|
---|---|
Chapter number | 13 |
Book title |
Genome Instability
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7306-4_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
Laura Baranello, Fedor Kouzine, Damian Wojtowicz, Kairong Cui, Keji Zhao, Teresa M. Przytycka, Giovanni Capranico, David Levens, Baranello, Laura, Kouzine, Fedor, Wojtowicz, Damian, Cui, Kairong, Zhao, Keji, Przytycka, Teresa M., Capranico, Giovanni, Levens, David |
Abstract |
Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological "breakome" of the DNA in human cells. |
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