Chapter title |
Characterization of Structural and Configurational Properties of DNA by Atomic Force Microscopy
|
---|---|
Chapter number | 37 |
Book title |
Genome Instability
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7306-4_37 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
Alice Meroni, Federico Lazzaro, Marco Muzi-Falconi, Alessandro Podestà |
Abstract |
We describe a method to extract quantitative information on DNA structural and configurational properties from high-resolution topographic maps recorded by atomic force microscopy (AFM). DNA molecules are deposited on mica surfaces from an aqueous solution, carefully dehydrated, and imaged in air in Tapping Mode. Upon extraction of the spatial coordinates of the DNA backbones from AFM images, several parameters characterizing DNA structure and configuration can be calculated. Here, we explain how to obtain the distribution of contour lengths, end-to-end distances, and gyration radii. This modular protocol can be also used to characterize other statistical parameters from AFM topographies. |
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