Chapter title |
Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions
|
---|---|
Chapter number | 39 |
Book title |
Genome Instability
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7306-4_39 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
Guðjón Ólafsson, Peter H. Thorpe, Ólafsson, Guðjón, Thorpe, Peter H. |
Abstract |
Artificially tethering two proteins or protein fragments together is a powerful method to query molecular mechanisms. However, this approach typically relies upon a prior understanding of which two proteins, when fused, are most likely to provide a specific function and is therefore not readily amenable to large-scale screening. Here, we describe the Synthetic Physical Interaction (SPI) method to create proteome-wide forced protein associations in the budding yeast Saccharomyces cerevisiae. This method allows thousands of protein-protein associations to be screened for those that affect either normal growth or sensitivity to drugs or specific conditions. The method is amenable to proteins, protein domains, or any genetically encoded peptide sequence. |
X Demographics
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 30% |
Researcher | 3 | 30% |
Lecturer > Senior Lecturer | 1 | 10% |
Student > Master | 1 | 10% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 60% |
Veterinary Science and Veterinary Medicine | 1 | 10% |
Agricultural and Biological Sciences | 1 | 10% |
Physics and Astronomy | 1 | 10% |
Unknown | 1 | 10% |