Chapter title |
Telomerase RNA Imaging in Budding Yeast and Human Cells by Fluorescent In Situ Hybridization
|
---|---|
Chapter number | 27 |
Book title |
Genome Instability
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7306-4_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
David Guérit, Maxime Lalonde, Pascal Chartrand |
Abstract |
Telomerase, the enzyme that elongates telomeres in most eukaryotes, is a ribonucleoprotein complex composed of a reverse transcriptase catalytic subunit (TERT in human, Est2 in the budding yeast S. cerevisiae), regulatory factors and a noncoding RNA called hTERC (in human) or TLC1 (in budding yeast). Telomerase trafficking is a major process in the biogenesis and regulation of telomerase action at telomeres. Due to its higher signal-to-noise ratio, imaging of the telomerase RNA moiety is frequently used to determine telomerase intracellular localization. Here we describe how to image telomerase RNA in human and yeast cells using fluorescence in situ hybridization. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 67% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Computer Science | 1 | 33% |
Medicine and Dentistry | 1 | 33% |