Chapter title |
Detection of DNA-RNA Hybrids In Vivo
|
---|---|
Chapter number | 24 |
Book title |
Genome Instability
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7306-4_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
María García-Rubio, Sonia I. Barroso, Andrés Aguilera |
Abstract |
DNA-RNA hybrids form naturally during essential cellular functions such as transcription and replication. However, they may be an important source of genome instability, a hallmark of cancer and genetic diseases. Detection of DNA-RNA hybrids in cells is becoming crucial to understand an increasing number of molecular biology processes in genome dynamics and function and to identify new factors and mechanisms responsible for disease in biomedical research. Here, we describe two different procedures for the reliable detection of DNA-RNA hybrids in the yeast Saccharomyces cerevisiae and in human cells: DNA-RNA Immunoprecipitation (DRIP) and Immunofluorescence. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 42 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 26% |
Student > Master | 7 | 17% |
Student > Doctoral Student | 4 | 10% |
Student > Ph. D. Student | 3 | 7% |
Professor > Associate Professor | 3 | 7% |
Other | 7 | 17% |
Unknown | 7 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 26 | 62% |
Agricultural and Biological Sciences | 5 | 12% |
Computer Science | 1 | 2% |
Immunology and Microbiology | 1 | 2% |
Engineering | 1 | 2% |
Other | 0 | 0% |
Unknown | 8 | 19% |