Chapter title |
The CellClamper: A Convenient Microfluidic Device for Time-Lapse Imaging of Yeast
|
---|---|
Chapter number | 36 |
Book title |
Genome Instability
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7306-4_36 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
Gregor W. Schmidt, Olivier Frey, Fabian Rudolf |
Abstract |
Time-lapse fluorescence imaging of yeast cells allows the study of multiple fluorescent targets in single cells, but is often hampered by the tedious cultivation using agar pads or glass bottom wells. Here, we describe the fabrication and operation of a microfluidic device for long-term imaging of yeast cells under constant or changing media conditions. The device allows acquisition of high quality images as cells are fixed in a two-dimensional imaging plane. Four yeast strains can be analyzed simultaneously over several days while up to four different media can be flushed through the chip. The microfluidic device does not rely on specialized equipment for its operation. To illustrate the use of the chip in DNA damage research, we show how common readouts for DNA damage or genomic instability behave upon induction with genotoxic chemicals (MMS, HU) or induction of a single double-strand break using induced CRISPR-Cas9 expression. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 19% |
Student > Master | 3 | 14% |
Student > Doctoral Student | 2 | 10% |
Student > Postgraduate | 2 | 10% |
Student > Bachelor | 2 | 10% |
Other | 4 | 19% |
Unknown | 4 | 19% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 33% |
Unspecified | 1 | 5% |
Chemistry | 1 | 5% |
Unknown | 4 | 19% |