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Mendeley readers
Chapter title |
Measuring the Levels of Ribonucleotides Embedded in Genomic DNA
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Chapter number | 22 |
Book title |
Genome Instability
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7306-4_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7305-7, 978-1-4939-7306-4
|
Authors |
Alice Meroni, Giulia M. Nava, Sarah Sertic, Paolo Plevani, Marco Muzi-Falconi, Federico Lazzaro |
Abstract |
Ribonucleotides (rNTPs) are incorporated into genomic DNA at a relatively high frequency during replication. They have beneficial effects but, if not removed from the chromosomes, increase genomic instability. Here, we describe a fast method to easily estimate the amounts of embedded ribonucleotides into the genome. The protocol described is performed in Saccharomyces cerevisiae and allows us to quantify altered levels of rNMPs due to different mutations in the replicative polymerase ε. However, this protocol can be easily applied to cells derived from any organism. |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 27% |
Student > Master | 4 | 27% |
Researcher | 3 | 20% |
Professor | 1 | 7% |
Unknown | 3 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 47% |
Agricultural and Biological Sciences | 4 | 27% |
Unknown | 4 | 27% |