Chapter title |
Single yeast cell imaging.
|
---|---|
Chapter number | 7 |
Book title |
Yeast Genetics
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1363-3_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1362-6, 978-1-4939-1363-3
|
Authors |
Heimo Wolinski, Sepp D Kohlwein, Sepp D. Kohlwein, Wolinski, Heimo, Kohlwein, Sepp D. |
Abstract |
Microscopic imaging techniques play a pivotal role in the life sciences. Here we describe labeling and imaging methods for live yeast cell imaging. Yeast is an excellent reference organism for biomedical research to investigate fundamental cellular processes, and has gained great popularity also for large-scale imaging-based screens. Methods are described to label live yeast cells with organelle-specific fluorescent dyes or GFP-tagged proteins, and how cells are maintained viable over extended periods of time during microscopy. We point out common pitfalls and potential microscopy artifacts arising from inhomogeneous labeling and depending on cellular physiology. Application and limitation of bleaching techniques to address dynamic processes in the yeast cell are described. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 24% |
Student > Master | 3 | 18% |
Researcher | 3 | 18% |
Student > Bachelor | 2 | 12% |
Professor | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 41% |
Biochemistry, Genetics and Molecular Biology | 4 | 24% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Chemistry | 1 | 6% |
Unknown | 4 | 24% |