| Chapter title |
Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver
|
|---|---|
| Chapter number | 16 |
| Book title |
Alpha-1 Antitrypsin Deficiency
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7163-3_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7161-9, 978-1-4939-7163-3
|
| Authors |
Marcela Aparicio-Vergara, Michaela Tencerova, Cecilia Morgantini, Emelie Barreby, Myriam Aouadi |
| Abstract |
Liver perfusion is a common technique used to isolate parenchymal and non-parenchymal liver cells for in vitro experiments. This method allows hepatic cells to be separated based on their size and weight, by centrifugation using a density gradient. To date, other methods allow the isolation of only one viable hepatic cellular fraction from a single mouse; either parenchymal (hepatocytes) or non-parenchymal cells (i.e., Kupffer cells or hepatic stellate cells). Here, we describe a method to isolate both hepatocytes and Kupffer cells from a single mouse liver, thereby providing the unique advantage of studying different liver cell types that have been isolated from the same organism. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 20% |
| Researcher | 6 | 12% |
| Student > Master | 6 | 12% |
| Student > Bachelor | 5 | 10% |
| Student > Postgraduate | 3 | 6% |
| Other | 5 | 10% |
| Unknown | 14 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 16% |
| Immunology and Microbiology | 7 | 14% |
| Biochemistry, Genetics and Molecular Biology | 6 | 12% |
| Medicine and Dentistry | 6 | 12% |
| Agricultural and Biological Sciences | 3 | 6% |
| Other | 2 | 4% |
| Unknown | 17 | 35% |