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Mendeley readers
Chapter title |
Methods to Identify and Characterize siRNAs Targeting Serpin A1 In Vitro and In Vivo Using RNA Interference
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Chapter number | 11 |
Book title |
Alpha-1 Antitrypsin Deficiency
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7163-3_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7161-9, 978-1-4939-7163-3
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Authors |
Stuart Milstein, Kun Qian, Linying Zhang, Alfica Sehgal |
Abstract |
RNAi is a powerful tool that can be used to probe gene function as well as for therapeutic intervention. We describe a workflow and methods to identify screen and select potent and specific siRNAs in vitro and in vivo using qPCR-based methods as well as an AAT activity assay. We apply these techniques to a set of siRNAs targeting rat AAT, and use this set to exemplify the cell-based and in vivo data that can be generated using these methods. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 33% |
Professor > Associate Professor | 1 | 33% |
Other | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Engineering | 1 | 33% |