| Chapter title |
Quantification of Total Human Alpha-1 Antitrypsin by Sandwich ELISA
|
|---|---|
| Chapter number | 20 |
| Book title |
Alpha-1 Antitrypsin Deficiency
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7163-3_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7161-9, 978-1-4939-7163-3
|
| Authors |
Qiushi Tang, Alisha M. Gruntman, Terence R. Flotte |
| Abstract |
In this chapter we describe an enzyme-linked immunosorbent assay (ELISA) to quantitatively measure human alpha-1 antitrypsin (AAT) protein levels in serum, other body fluids or liquid media. This assay can be used to measure the expression of the human AAT (hAAT) gene in a variety of gene transfer or gene downregulation experiments.A hAAT-specific capture antibody and a HRP-conjugated anti-AAT detection antibody are used in this assay. The conjugated anti-AAT used in this protocol, instead of the typical sandwich which employs an unconjugated antibody followed by a specifically conjugated IgG, makes the assay simpler and decreases variability. This provides a useful tool to evaluate the AAT levels in clinical and research samples and can allow fairly rapid testing of a large number of samples. |
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Demographic breakdown
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|---|---|---|
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| Unknown | 1 | 25% |
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