| Chapter title |
Quantification of Z-AAT by a Z-Specific “Sandwich” ELISA
|
|---|---|
| Chapter number | 22 |
| Book title |
Alpha-1 Antitrypsin Deficiency
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7163-3_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7161-9, 978-1-4939-7163-3
|
| Authors |
Florie Borel, Qiushi Tang, Christian Mueller |
| Abstract |
This protocol describes an enzyme-linked immunosorbent assay (ELISA) to specifically detect Z-alpha-1 antitrypsin (AAT), the most common protein variant associated with alpha-1 antitrypsin deficiency. This "sandwich" ELISA relies on an anti-Z-AAT specific capture antibody and a HRP-conjugated anti-AAT detection antibody. This method would be of interest to identify and quantify Z-AAT in a variety of samples such as cell culture medium, cell or tissue lysate, animal or patient serum. Because this method is specific and sensitive, it would be particularly valuable for detection of Z-AAT in the presence of background M-AAT, for instance when quantifying silencing of Z-AAT in patients undergoing M-AAT augmentation therapy. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 30% |
| Unspecified | 1 | 10% |
| Professor > Associate Professor | 1 | 10% |
| Student > Postgraduate | 1 | 10% |
| Unknown | 4 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Unknown | 6 | 60% |