Chapter title |
Quantitative RT-PCR for MicroRNAs in Biofluids
|
---|---|
Chapter number | 21 |
Book title |
Drug Safety Evaluation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7172-5_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7170-1, 978-1-4939-7172-5
|
Authors |
Michael Thorsen, Thorarinn Blondal, Peter Mouritzen |
Abstract |
MicroRNAs in biofluids hold great promise as minimally invasive diagnostic biomarkers for a wide range of diseases and biological processes. One of the most sensitive technologies for detection and measuring expression levels of microRNA is quantitative RT-PCR. However, quantification of microRNA in biofluid samples is challenging in many ways. Biofluids contain low levels of RNA and high levels of inhibitors of enzymatic processes like reverse transcription and PCR. Furthermore, biofluids are susceptible to many preanalytical variables. Here we describe procedures developed to address these challenges, which include highly sensitive and accurate microRNA detection methods, combined with optimized protocols for sample handling and preparation, and extensive quality control (QC) procedures. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 21% |
Student > Bachelor | 2 | 14% |
Student > Master | 2 | 14% |
Lecturer | 1 | 7% |
Student > Ph. D. Student | 1 | 7% |
Other | 3 | 21% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 14% |
Medicine and Dentistry | 2 | 14% |
Unspecified | 1 | 7% |
Immunology and Microbiology | 1 | 7% |
Other | 3 | 21% |
Unknown | 3 | 21% |