Chapter title |
The Use of Bacterial Repair Endonucleases in the Comet Assay
|
---|---|
Chapter number | 9 |
Book title |
Drug Safety Evaluation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7172-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7170-1, 978-1-4939-7172-5
|
Authors |
Andrew R. Collins |
Abstract |
The comet assay is a sensitive electrophoretic method for measuring DNA breaks at the level of single cells, used widely in genotoxicity experiments, in biomonitoring, and in fundamental research. Its sensitivity and range of application are increased by the incorporation of an extra step, after lysis of agarose-embedded cells, in which the DNA is digested with lesion-specific endonucleases (DNA repair enzymes of bacterial or phage origin). Enzymes with specificity for oxidized purines, oxidized pyrimidines, alkylated bases, UV-induced cyclobutane pyrimidine dimers, and misincorporated uracil have been employed. The additional enzyme-sensitive sites, over and above the strand breaks detected in the standard comet assay, give a quantitative estimate of the number of specific lesions present in the cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 30% |
Student > Master | 4 | 17% |
Researcher | 3 | 13% |
Student > Postgraduate | 2 | 9% |
Student > Doctoral Student | 2 | 9% |
Other | 1 | 4% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 26% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 17% |
Agricultural and Biological Sciences | 4 | 17% |
Chemistry | 2 | 9% |
Unspecified | 1 | 4% |
Other | 2 | 9% |
Unknown | 4 | 17% |