Chapter title |
Tissue Sampling and Processing for Histopathology Evaluation
|
---|---|
Chapter number | 4 |
Book title |
Drug Safety Evaluation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7172-5_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7170-1, 978-1-4939-7172-5
|
Authors |
Mohamed Slaoui, Anne-Laure Bauchet, Laurence Fiette |
Abstract |
Histological procedures aim at providing good-quality sections that can be used for a light microscopic evaluation of tissue. These are applicable to identify either spontaneous or diseases-induced changes. Routinely, tissues are fixed with neutral formalin 10%, embedded in paraffin, and manually sectioned with a microtome to obtain 4-5 μm thick paraffin sections. Dewaxed sections are then stained with HE&S (hematoxylin-eosin and saffron) or can be used for other purposes (special stains, immunohistochemistry, in situ hybridization, etc.). During this processing, many steps and procedures are critical to ensure standard and interpretable sections. This chapter provides key recommendations to efficiently achieve this objective. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 88 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 15 | 17% |
Student > Master | 10 | 11% |
Student > Ph. D. Student | 5 | 6% |
Student > Doctoral Student | 3 | 3% |
Researcher | 3 | 3% |
Other | 7 | 8% |
Unknown | 45 | 51% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 18 | 20% |
Biochemistry, Genetics and Molecular Biology | 11 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 6% |
Veterinary Science and Veterinary Medicine | 2 | 2% |
Nursing and Health Professions | 2 | 2% |
Other | 6 | 7% |
Unknown | 44 | 50% |