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Mendeley readers
| Chapter title |
Principles and Methods of Immunohistochemistry
|
|---|---|
| Chapter number | 5 |
| Book title |
Drug Safety Evaluation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7172-5_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7170-1, 978-1-4939-7172-5
|
| Authors |
José A. Ramos-Vara |
| Abstract |
Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and the reagents used. Pretreatments such as blocking steps for endogenous activities and antigen retrieval are included. Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized. |
Mendeley readers
The data shown below were compiled from readership statistics for 339 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | <1% |
| Jordan | 1 | <1% |
| South Africa | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 335 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 68 | 20% |
| Student > Master | 45 | 13% |
| Student > Ph. D. Student | 31 | 9% |
| Researcher | 23 | 7% |
| Student > Doctoral Student | 14 | 4% |
| Other | 28 | 8% |
| Unknown | 130 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 57 | 17% |
| Medicine and Dentistry | 51 | 15% |
| Agricultural and Biological Sciences | 30 | 9% |
| Neuroscience | 13 | 4% |
| Immunology and Microbiology | 10 | 3% |
| Other | 40 | 12% |
| Unknown | 138 | 41% |