Chapter title |
Protocol for Directed Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) to a Hepatic Lineage
|
---|---|
Chapter number | 15 |
Book title |
Alpha-1 Antitrypsin Deficiency
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7163-3_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7161-9, 978-1-4939-7163-3
|
Authors |
Kaserman, Joseph E., Wilson, Andrew A., Joseph E. Kaserman, Andrew A. Wilson |
Abstract |
Directed differentiation is a powerful cell culture technique where developmental pathways are applied to a pluripotent progenitor in order to generate specific terminally differentiated cell populations. Here, we describe a serum-free protocol using growth factors in defined concentrations to derive iPSC-hepatic cells starting from both feeder and feeder-free conditions. The generated iPSC-hepatic cells are developmentally similar to fetal stage hepatocytes, and when generated from patients with genetic mutations such as alpha-1 antitrypsin deficiency recapitulate pathologic changes associated with clinical disease, such as protein misfolding, intracellular retention of misfolded proteins, and elevated levels of ER stress. |
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