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Western Blotting

Overview of attention for book
Cover of 'Western Blotting'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Early Days of Blotting
  3. Altmetric Badge
    Chapter 2 Origins of Protein Blotting
  4. Altmetric Badge
    Chapter 3 Western Blotting: Remembrance of Things Past
  5. Altmetric Badge
    Chapter 4 Simian Virus 40 and Protein Transfer
  6. Altmetric Badge
    Chapter 5 Western Blotting: An Introduction
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    Chapter 6 From Little Helpers to Automation
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    Chapter 7 Spectrophotometric Methods to Determine Protein Concentration
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    Chapter 8 Solubilization of proteins: the importance of lysis buffer choice.
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    Chapter 9 Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
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    Chapter 10 Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel
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    Chapter 11 Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel
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    Chapter 12 Slice Blotting
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    Chapter 13 Localizing Proteins by Tissue Printing
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    Chapter 14 Dot-Immunobinding Assay (Dot-Iba)
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    Chapter 15 Analysis of Antibody Clonotype by Affinity Immunoblotting
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    Chapter 16 Glycosaminoglycan Blotting and Detection After Electrophoresis Separation
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    Chapter 17 A well-based reverse-phase protein array of formalin-fixed paraffin-embedded tissue.
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    Chapter 18 Quantitative Computerized Western Blotting in Detail
  20. Altmetric Badge
    Chapter 19 Cationic Electrophoresis and Eastern Blotting
  21. Altmetric Badge
    Chapter 20 A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies
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    Chapter 21 Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting.
  23. Altmetric Badge
    Chapter 22 Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting
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    Chapter 23 Multistrip Western blotting: a tool for comparative quantitative analysis of multiple proteins.
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    Chapter 24 Western Blotting Using PVDF Membranes and Its Downstream Applications
  26. Altmetric Badge
    Chapter 25 Blotting from PhastGel to Membranes by Ultrasound
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    Chapter 26 Western Blotting of High and Low Molecular Weight Proteins Using Heat
  28. Altmetric Badge
    Chapter 27 Membrane Strip Affinity Purification of Autoantibodies
  29. Altmetric Badge
    Chapter 28 Strip Immunoblotting of Multiple Antigenic Peptides
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    Chapter 29 Double-Blotting: A Solution to the Problem of Nonspecific Binding of Secondary Antibodies in Immunoblotting Procedures
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    Chapter 30 Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis
  32. Altmetric Badge
    Chapter 31 Immunodetection of P-Selectin Using an Antibody to Its C-Terminal Tag
  33. Altmetric Badge
    Chapter 32 Improvements and Variants of the Multiple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening
  34. Altmetric Badge
    Chapter 33 Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates
  35. Altmetric Badge
    Chapter 34 Immunoprecipitation: Western Blot for Proteins of Low Abundance
  36. Altmetric Badge
    Chapter 35 Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications
  37. Altmetric Badge
    Chapter 36 Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein–DNA Complexes
  38. Altmetric Badge
    Chapter 37 Grid-Immunoblotting
  39. Altmetric Badge
    Chapter 38 Detection and Quantification of Protein–Protein Interactions by Far-Western Blotting
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    Chapter 39 Western Blot Analysis of Adhesive Interactions Under Fluid Shear Conditions: The Blot Rolling Assay
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    Chapter 40 Centrifuge Blotting
  42. Altmetric Badge
    Chapter 41 Blotting of Coomassie Blue-Stained Proteins from PAGE Gels to Transparencies
  43. Altmetric Badge
    Chapter 42 B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells
  44. Altmetric Badge
    Chapter 43 T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells
  45. Altmetric Badge
    Chapter 44 Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays
  46. Altmetric Badge
    Chapter 45 SDS-PAGE to Immunoblot in One Hour
  47. Altmetric Badge
    Chapter 46 Single-cell Western blotting.
  48. Altmetric Badge
    Chapter 47 Protein Detection by Simple Western™ Analysis
  49. Altmetric Badge
    Chapter 48 Western Blotting Using Microfluidics
  50. Altmetric Badge
    Chapter 49 Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis
  51. Altmetric Badge
    Chapter 50 Fingerprint Deposition on Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase
  52. Altmetric Badge
    Chapter 51 Other Notable Protein Blotting Methods: A Brief Review
Attention for Chapter 7: Spectrophotometric Methods to Determine Protein Concentration
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

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Citations

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Chapter title
Spectrophotometric Methods to Determine Protein Concentration
Chapter number 7
Book title
Western Blotting
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2694-7_7
Pubmed ID
Book ISBNs
978-1-4939-2693-0, 978-1-4939-2694-7
Authors

J. P. Dean Goldring, Goldring, J. P. Dean

Editors

Biji T. Kurien, R. Hal Scofield

Abstract

Measuring the concentration of proteins is an essential part of enzyme evaluations or to monitor protein yields during protein isolation procedures. Decisions on the usefulness of any isolation procedure depend on knowing the relative concentrations of a particular protein or enzyme in relation to the concentrations of all the proteins present. Protein concentration in solution is generally measured with spectrophotometry in the UV range or in the presence of dyes or copper interacting with the protein. This review describes protein determination methods for measuring protein concentration in solution.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 11 29%
Student > Ph. D. Student 5 13%
Student > Postgraduate 2 5%
Student > Master 2 5%
Student > Doctoral Student 1 3%
Other 2 5%
Unknown 15 39%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 16%
Biochemistry, Genetics and Molecular Biology 4 11%
Chemistry 3 8%
Nursing and Health Professions 2 5%
Veterinary Science and Veterinary Medicine 2 5%
Other 3 8%
Unknown 18 47%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 February 2020.
All research outputs
#4,999,684
of 23,924,386 outputs
Outputs from Methods in molecular biology
#1,455
of 13,498 outputs
Outputs of similar age
#69,357
of 358,728 outputs
Outputs of similar age from Methods in molecular biology
#110
of 988 outputs
Altmetric has tracked 23,924,386 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,498 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 358,728 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 988 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.